1) Different breeds of cows and buffaloes and their production and reproduction parameters
2) Conservation and development of indigenous breeds through selective breeding.
3) Benefits of Crossbreeding and genetic improvement of dairy animals
4) The existing State Breeding Policy and its enforcement.
5) Introduction to AI, and its importance, role of AI in genetic up gradation across nations, Natural Service (NS) vs. AI, advantages and limitations.
6) External and internal body parts of a dairy animal and their function
7) Male reproductive organs & their functions
8) Semen, its collection, evaluation, processing, preservation
• Different types of semen packing,
• Structure of mini and medium straws
• Information printed on straw and its importance
• Breed wise Straw color codes
9) Female reproductive organs & their functions
10) Estrus cycle:
• Internal and external symptoms at different stages of estrus cycle
• Correct time of insemination
• Determinants of first AI in heifers
• Methods of heat detection in cattle and buffaloes
11) Normal reproductive cycle
12) Puberty, Maturity, Breeding, Fertilization, Implantation, Gestation and Calving
13) Ideal calving interval• Service period, dry period and Inter-calving period
14) Process of insemination:
• Collecting History
• Standard Operating Procedure (SOP)
15) Importance of:
• Proper method of semen withdrawal from container
• Proper thawing
• Proper preparation of AI gun
• Proper site of semen deposition
• Care of animal during & after insemination
16) AI equipment and accessories & their care
17) Liquid nitrogen handling:
• Structure of LN container
• Handling & care of LN container
• Precaution in handling of LN2
• Different models of LN containers
• Importance of maintaining cold chain and LN refilling schedule.
• Proper LN level in container & its checking. Evaporation rates and refilling interval of commonly used containers in the field under normal working conditions.
• LN conservation measures
18) Pregnancy Diagnosis
19) Methods of calculating conception rates and factors affecting conception rates
20) Method of drying of animals on completion of 7th month pregnancy.
21) Common reproductive disorders/ diseases, repeat breeding, causes of abortion, etc.
22) Measures to obtain maximum fertility
23) Ear tagging, importance of record keeping, recording formats and submission of records into the INAPH application (offline & online versions) through
24) PDA/Net book/Desktop (training in data entry with dummy data on test server, different flash messages, saving the data, synchronization of data with the server and using action reports in day to day work.
25) Starting an AI centre
26) Method of non-surgical castration
27) Care and management of new born calf and heifers till it becomes pregnant at farmers perception.
28) Care and management of Dry Pregnant animals
29) Care and management of animals before and after calving, precautions at the time of calving and use of naval kit for disinfection of naval cord
30) Importance of Animal housing and general management in getting full expression of genetic capability
31) Importance of bio-security measures to be adopted during AI.
32) Economically important diseases and their prevention through timely vaccination; various available vaccines; vaccination schedules; importance of maintaining cold chain
33) Basic aspects of nutrition and concept of Ration Balancing
34) Importance of proper nutrition including feeding of vitamins and mineral mixtures and deworming  in fertility management with emphasis on the adverse impact of macro and micronutrients deficiencies on fertility status/reproductive health of animal.
35) Vaccination schedule for FMD, HS, BQ, Brucellosis and Anthrax (in Karnataka and Assam)
36) Veterinary first aid
37) Hygiene clean milk production and prevention of mastitis
38) Importance of Animal Insurance; various insurance schemes
39) Various government schemes in the dairy sector: DEDS, NPBBD and NDP